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Biological Sciences : Therapeutics
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Immortalized Human Implantation Site Intermediate Trophoblastic Cell Line, IST-1 (JHU Ref 3408)
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Human HPV-16 E6E7 transduced immortal implantation site cell line was established from trophoblastic cells that grew out from chorionic explants of cultured fresh human placental tissue. This IST-1 cell line was obtained from a single clone of HPV-16 E6E7 transduced trophoblastic cells. IST-1 is positive for Mel-CAM, or CD146, a membrane glycoprotein that functions as a Ca 2+-independent molecule involved in heterophilic cell-cell interactions. This cell line is useful in the study of the role of Mel-CAM in the interaction between the implantation site intermediate trophoblastic cells and uterine smooth muscle cells and the biological mechanisms in the implantation microenvironment in normal and possibly pathologic conditions.
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Inventors
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le-Ming Shih, M.D., Ph.D. & Robert J. Kurman, M.D.
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Keywords
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Research Tool, animal model, antibody, cell line, culture, directed evolution, DNA probe, DNA/RNA sequencing, DNA/RNA synthesis, electrophoresis, elisa, enzyme, equipment
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Patents
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No Patent Filed
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Reference
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Shih I., Wang T., Wu T., Kurman R.J., Gearhart J.D. Expression of Mel-CAM in implantation site intermediate trophoblastic cell line, IST-1, limits its migration on uterine smooth muscle cells. J Cell Sci 1998 Sep;111 ( Pt 17):2655-64
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Commercial Uses
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This cell line is valuable in the research of cell biology, immunology and pathology of human implantation site intermediate (extravillous) trophoblasts which is fundamental for the understanding of normal human implantation and a variety of trophoblast-related disorders including spontaneous abortion, pre-eclampsia, and gestational trophoblastic diseases.
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Applicable Categories
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Biological Sciences
» Therapeutics
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Contact
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Jacqueline Walker
The Johns Hopkins University
100 North Charles Street, 5th Floor
Baltimore, Maryland 21201
Email: jwalke50@jhmi.edu
Phone:410-516-8300
Fax: 410-516-4411
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